Indeever Madireddy and his aquarium Indeever Madireddy
A 17-year-old high school student in California has sequenced the genome of the freshwater angelfish (Pterophyllum scalare) for the first time.
Indeever Madireddy sequenced the genome of his pet angelfish Calvin after it died in March this year. 鈥淎lthough my fish was dead, I wanted to preserve it forever,鈥 he says. 鈥淪o I decided to sequence the genome of the angelfish with the hopes that I could contribute that information to the scientific community, while also paying a small tribute to my pet!鈥
Madireddy did the sequencing at , a community lab in Santa Clara, California, that makes advanced equipment available to anyone for a small membership fee.
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He stored his fish at -80掳C at the lab to preserve its DNA. He spent around a month preparing and learning how to sequence it.
The sequencing was done over two weekends with a small sequencer made by Oxford Nanopore. These sequencers read off the sequence of DNA molecules as they pass through tiny pores.
Nanopore sequencers can sequence much longer individual pieces of DNA than competing methods. This makes it easier for sequencing software to assemble the genome by putting all these pieces back together 鈥 like a jigsaw puzzle with bigger and fewer pieces.
Analysing the data took another two months and a lot more learning, says Madireddy. The sequence data has been put on 聽and a short paper describing the work was published on 18 October.
Angelfish in an aquarium H. Schmidbauer/mauritius images GmbH/Alamy
The angelfish is native to the Amazon basin and has become a popular aquarium fish worldwide. Its genome hasn’t revealed any particular surprises, being similar to those of related fish.
The sequencing cost around $2000, says Madireddy, of which he managed to raise .
鈥淭he most important part of this community lab was the people I met there,鈥 says Madireddy. 鈥淭wo people in particular, Johan Sosa and Kurt Chang, were of huge support to me.鈥
鈥淭his is a wonderful example of an inquisitive spirit and what young scientists can do when you remove technology barriers like cost and complexity,鈥 says , CEO of Oxford Nanopore.
Madireddy is a keen fish-keeper and biologist, and has previously done work using CRISPR gene editing. He had raised Calvin from the egg stage along with other hatchlings.
鈥淭he rest of the angelfish are still happy and healthy in my aquarium,鈥 he says.
microPublication Biology
Article amended on 28 October 2022
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